Scientific Publications

I.D. Nordhorn, D. Dietrich, C. Verlemann, A. Vennemann, R. Schmid, M. Elinkmann, J. Fuchs, M. Sperling, M. Wiemann, U. Karst: Spatially and size-resolved analysis of gold nanoparticles in rat spleen after intratracheal instillation by laser ablation-inductively coupled plasma-mass spectrometry. Metallomics, 2021 13(6) mfab028. DOI: 10.1093/mtomcs/mfab028

O. Reifschneider, A. Vennemann, G. Buzanich, M. Radtke, U. Reinholz, H. Riesemeier, J. Hogeback, C. Köppen, M. Großgarten, M. Sperling, M. Wiemann, U. Karst: Revealing Silver Nanoparticle Uptake by Macrophages Using SR-μXRF and LA-ICP-MS. Chem Res Toxicol, 2020 May 18;33(5):1250-1255. DOI: 10.1021/acs.chemrestox.9b00507

M. Wiemann , A. Vennemann, C. Venzago, G.-G. Lindner, T. B. Schuster, N. Krueger: Serum Lowers Bioactivity and Uptake of Synthetic Amorphous Silica by Alveolar Macrophages in a Particle Specific Manner. Nanomaterials (Basel) 2021 Mar 3;11(3):628 DOI: 10.3390/nano11030628

S. Seiffert, A. Vennemann, I. Nordhorn, S. Kröger, M. Wiemann, U. Karst. LA ICP MS and immunohistochemical staining with lanthanide-labeled antibodies to study the uptake of CeO2 nanoparticles by macrophages in tissue sections. Chemical Research in Toxicology 2022 35 (6), 981-991 DOI: 10.1021/acs.chemrestox.1c00433

K. M. Eder, A. Marzi, Á. Barroso, S. Ketelhut, B. Kemper, J. Schnekenburger, "Label free Digital Holographic Microscopy for in vitro Cyto-toxicity Effect Quantification of Organic Nanoparticles", Cells 11, 644 (2022). DOI: 10.3390/cells11040644

 

Conference Proceedings

A. Marzi, I. Nordhorn, K. Eder, A. Vennemann, M. Wiemann, U. Karst, B. Kemper, J. Schnekenburger , "Digital holographic microscopy complements mass spectrometry for label-free quantification of nanoparticles within single cells" (DH2022, in press).

K. Eder, A. Marzi, M. Wiemann, U. Rauen, Björn Kemper, Jürgen Schnekenburger , "Morphological alterations in primary hepatocytes upon nanomaterial incubation assessed by digital holographic microscopy and holotomography", Proc. SPIE 11970, 119700H (2022). DOI: 10.1117/12.2610171

A. Ntovas, Á. Barroso, K. Eder, S. Ketelhut, A. Vennemann, M. Wiemann, J. Schnekenburger, B. Kemper , "Utilizing quantitative phase imaging with digital holographic microscopy for characterization of nanomaterial-induced tissue alterations", Proc. SPIE 11970, 119700D (2022). DOI: 10.1117/12.2609625

A. Marzi, I. Nordhorn, K. Eder, M. Wiemann, U. Karst, B. Kemper, J. Schnekenburger, "Label-free identification and quantification of nanoparticles in single cells by combining digital holographic microscopy and mass spectrometry", Proc. SPIE 11972, 1197209 (2022). DOI: 10.1117/12.2609700

T. Kutscher, K. Eder, A. Marzi, Á. Barroso, J. Schnekenburger, B. Kemper, "Cell Detection and Segmentation in Quantitative Digital Holographic Phase Contrast Images Utilizing a Mask Region-based Convolutional Neural Network", (DH2021, virtuell). DOI: 10.1364/AIS.2021.JTu5A.23

A. Ntovas, Á. Barroso, K. Eder, A. Vennemann, M. Wiemann, J. Schnekenburger, B. Kemper, "Quantification of Nanoparticle-mediated Alterations in Histological Tissue Sections Utilizing Quantitative Digital Holographic Phase Microscopy" (DH2021, virtuell). DOI: 10.1364/DH.2021.DW5C.4

B. Kemper, Á. Barroso, K. Eder, A. Marzi, S. Ritz, A. Ntovas, J. Schnekenburger, S. Ketelhut, "Enhanced quantitative phase imaging in Mach-Zehnder interferometer-based digital holographic microscopy by modulation of the object illumination with an electrically focus tunable lens", Proc. SPIE 11786, 117860I (2021). DOI: 10.1117/12.2592534

B. Kemper, Á. Barroso, K. Eder, A. Marzi, S. Ritz, J. Schnekenburger, S. Ketelhut, "Reduction of the influence of parasitic interferences and coherence induced disturbances in digital holography-based quantitative phase microscopy by modulation of the object illumination with an electrically focus tunable lens", Proc. SPIE 11653, 116530A (2021). DOI: 10.1117/12.2577807

B. Kemper, H. Eilers, T. Klein, K. Brinker, S. Ketelhut, "Quantitative phase imaging-based machine learning approaches for the analysis of adherent and suspended cells", Proc. SPIE11649, 116490B (2021). DOI: 10.1117/12.2577825

K. Eder, T. Kutscher, A. Marzi, Á. Barroso, J. Schnekenburger, B. Kemper, "Automated detection of macrophages in quantitative phase images by deep learning using a Mask Region-based Convolutional Neural Network", Proc. SPIE 116551, 116551K (2021). DOI: 10.1117/12.2577232

 

Posters

D. Breitenstein, K. Lamann, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Combination of Thin Layer Chromatography and Secondary Ion Mass Spectrometry for the Analysis of Complex Sample Systems. ECASIA, Dresden, September 2019, Deutschland.

D. Breitenstein, K. Lamann, C. Brungs, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Analysis of Tattooed Human Skin Samples by ToF-SIMS and Orbitrap-SIMS. ECASIA, Dresden, September 2019, Deutschland.

D. Breitenstein, K. Lamann, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Combination of Thin Layer Chromatography and Secondary Ion Mass Spectrometry for the Analysis of Complex Sample Systems. SIMS, Oktober 2019, Kyoto, Japan.

D. Breitenstein, K. Lamann, C. Brungs, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Analysis of Tattooed Human Skin Samples by ToF-SIMS and Orbitrap-SIMS. DGMS, März 2020, Münster, Deutschland.

D. Breitenstein, K. Lamann, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Combination of Thin Layer Chromatography and Secondary Ion Mass Spectrometry for the Analysis of Complex Sample Systems. DGMS, März 2020, Münster, Deutschland.

D. Breitenstein, K. Lamann, A. Vennemann, B. Hagenhoff, M. Wiemann: Weiterentwicklung der ToF-SIMS Analytik an Zell- und Gewebeproben) NanoCare Online-Clustermeeting, 21.10.2021.

K. Eder, A. Marzi, M. Wiemann, U. Rauen, B. Kemper, J. Schnekenburger: Morphological alterations in primary hepatocytes upon nanomaterial incubation assessed by digital holographic microscopy and holotomography. NanoCare Online-Clustermeeting, 21.10.2021.

M. Elinkmann, U. Karst : A statistical model fit to extrapolate signal durations in the inductively coupled plasma-mass spectrometry in single nanoparticle mode. DGMS, März 2020, Münster, Deutschland.

K. Eder, A. Marzi, M. Wiemann, U. Rauen, Björn Kemper, Jürgen Schnekenburger, "Morphological alterations in primary hepatocytes upon nanomaterial incubation assessed by digital holographic microscopy and holotomography", Quantitative phase imaging conference, SPIE Photonics West, Februar 2022, virtuell.

K. Eder, T. Kutscher, A. Marzi, Á. Barroso, J. Schnekenburger, B. Kemper, "Automated detection of macrophages in quantitative phase images by deep learning using a Mask Region-based Convolutional Neural Network", Quantitative phase imaging conference, SPIE Photonics West, Februar 2021, virtuell.

M. Elinkmann, S. Reuter, D. Ahlers, A. Köhrer, C. Quarles Jr, M. Sperling, A. Vennemann, M. Hippler, M. Wiemann, U. Karst: Quantitative Elementanalytik von einzelnen Zellen mittels induktiv gekoppelter Plasma-Massenspektrometrie (AP4). NanoCare 4.0. Clustertreffen, September 2021, Virtuelles Meeting.

B. Kemper, A. Ntovas, Á. Barroso, K. Eder, S. Ketelhut, A. Vennemann, M. Wiemann, J. Schnekenburger: Analyse von Nanopartikel-induzierten Gewebeveränderungen mit quantitativer digitalholographischer Phasenkontrastmikroskopie. NanoCare Online-Clustermeeting, 21.10.2021.

A. Marzi, I. Nordhorn, K. Eder, M. Wiemann, U. Karst, B. Kemper, J. Schnekenburger: Label-free identification and quantification of nanoparticles in single cells by combining digital holographic microscopy and mass spectrometry. NanoCare Online-Clustermeeting, 21.10.2021

I. Nordhorn, A. Vennemann, M. Sperling, M. Wiemann, U. Karst: Laserablation mit induktiv gekoppelter Plasma-Massenspektrometrie zur Untersuchung von Goldnanopartikeln in Milz- und Nierengewebe. NanoCare 4.0. Clustertreffen, September 2019, Frankfurt a. M., Deutschland.

I. Nordhorn, J. Fuchs, A. Vennemann, M. Sperling, M. Wiemann, U. Karst: Investigation of the gold nanoparticle distribution in tissues by means of LA-ICP-MS and LA-spICP-MS. 7th International Symposium on Metallomics, Juli 2019, Warschau, Polen.

I. Nordhorn , A. Schubert, A. Vennemann, M. Wiemann, U. Karst : A multimodal imaging approach to investigate metallic nanoparticles in rat lung tissues.IMSI, November 2020, Virtuelles Meeting.

I. Nordhorn, C. Verlemann, A. Vennemann, M. Sperling, M. Wiemann, U. Karst: Investigating the in vivo gold nanoparticle translocation - Quantitative bioimaging and single particle analysis by means of LA-ICP-MS. DGMS, März 2020, Münster, Deutschland.

I. Nordhorn , A. Vennemann, M. Wiemann, U. Karst: Molekulares Bioimaging von Zellkulturen nach Exposition mit Nanomaterialien mittels matrixassistierter Laserdesorptions-/Ionisations-Massenspektrometrie. NanoCare 4.0. Clustertreffen, September 2021, Virtuelles Meeting.

I. Nordhorn, A. Vennemann, M. Wiemann, U. Karst: Molekulares Bioimaging von Zellkulturen nach Exposition mit Nanomaterialien mittels Matrix-assistierter Laser-Desorptions-/Ionisations-Massenspektrometrie. NanoCare Online-Clustermeetin, 21.10.2021

S. Seiffert, A. Vennemann, D. Dietrich, I. D. Nordhorn, M. Wiemann, U. Karst: LA‑ICP-MS and immunohistochemical staining with lanthanide-labeled antibodies to study the in vivo uptake of CeO2 nanoparticles by macrophages. NanoCare Clustertreffen 2021, online, 21.09.2021.

A. Vennemann, M. Wiemann, und die Mitglieder des NBQ-Konsortium: AP2: In vivo-Verteilung von Nanopartikeln: Identifikation Nanopartikel-haltiger Zellen in Blut und Geweben. NanoCare Clustermeeting, Frankfurt, 26.9.2019

A. Vennemann, S. Jafoui, U. Rauen, M. Wiemann: Etablierung primärer Kupffer-Zellkulturen zum Nachweis nanotoxischer Effekte (NanoBioQuant), NanoCare Online-Clustermeetin, 21.10.2021.

M. Wiemann, S. Gröters, B. Hagenhoff, J. Schnekenburger, U. Karst: Quantifizierung von Nanomaterialien im Gewebe für die regulatorische Analytik und die Entwicklung von in vitro-Methoden. NanoCare Clustermeeting, Frankfurt, 26.9.2019.

 

Talks at Scientific Conferences

D. Breitenstein, K. Lamann, C. Brungs, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Analysis of Tattooed Human Skin Samples by ToF-SIMS and Orbitrap-SIMS. IUVSTA-Workshop, Mai 2019, Zakopane, Polen.

A. Marzi, I. Nordhorn, K. Eder, A. Vennemann, M. Wiemann, U. Karst, B. Kemper, J. Schnekenburger, "Digital holographic microscopy complements mass spectrometry for abel-free quantification of nanoparticles within single cells", Optica Digital Holography and Three-Dimensional Imaging conference August 2022, London, Großbritannien (angenommen), virtuell.

A. Marzi, I. Nordhorn, K. Eder, M. Wiemann, U. Karst, B. Kemper, J. Schnekenburger, "Label-free identification and quantification of nanoparticles in single cells by combining digital holographic microscopy and mass spectrometry", Quantitative phase imaging conference, SPIE Photonics West On Demand, Februar 2022, virtuell.

I. Nordhorn , A. Vennemann, M. Wiemann, U. Karst : Analysis of silver and gold nanoparticles in tissue samples by means of laser ablation-single particle ICP-MS. 6. DAAS Doktorandenseminar, September 2021, Virtuelles Meeting.

I. Nordhorn , A. Vennemann, M. Sperling, M. Wiemann, U. Karst: Elemental and molecular bioimaging of nanoparticles and their impact on biological tissues. SciX Conference, Oktober 2021, Providence, USA/ Recorded Presentation.

M. Elinkmann, S. Reuter, A. Köhrer, C. Quarles Jr, M. Sperling, M. Hippler, U. Karst : Using inductively coupled plasma-mass spectrometry to investigate essential element distributions in single algal cells. DGMS Young Scientists 1st Digital Meeting, Juni 2021.

M. Elinkmann, S. Reuter, C. Quarles Jr, M. Sperling, M. Hippler, U. Karst : Single-cell ICP-MS Analysis of Essential Element Distributions in Chlamydomonas Reinhardtii Algae with Nutritional Iron Deficiency. SciX Conference, Oktober 2021, Providence, USA/ Recorded Presentation.

M. Elinkmann, A. Köhrer, S. Reuter, C. Quarles Jr, M. Sperling, M. Hippler, U. Karst : Investigation of Trace Elements in Single Cells Using Inductively Coupled Plasma - Mass Spectrometry with a Total Consumption Sample Introduction System. Winter Conference on Plasma Spectrochemistry, Januar 2022, Tucson AZ, USA.

B. Kemper : NanoBioQuant -Marker-freie optische Quantifizierung von Nanotoxizität , NanoCare‐Clustertreffen September 2019, Frankfurt, Deutschland.

B. Kemper, Á. Barroso, K. Eder, A. Marzi, S. Ritz, A. Ntovas, J. Schnekenburger, S. Ketelhut, "Enhanced quantitative phase imaging in Mach-Zehnder interferometer-based digital holographic microscopy by modulation of the object illumination with an electrically focus tunable lens", SPIE Optical Metrology, Juni 2021, virtuell.

B. Kemper, Á. Barroso, K. Eder, A. Marzi, S. Ritz, A. Ntovas, J. Schnekenburger, S. Ketelhut, "Reduction of coherence induced disturbances in quantiative phase imaging with laser-based Mach-Zehnder interferometer digital holographic microscopy utilizing an electrically focus tunable lens", Focus on Microscopy, März 2021, virtuell

B. Kemper, Á. Barroso, K. Eder, A. Marzi, S. Ritz, J. Schnekenburger, S. Ketelhut, "Reduction of the influence of parasitic interferences and coherence induced disturbances in digital holography-based quantitative phase microscopy by modulation of the object illumination with an electrically focus tunable lens", Quantitative phase imaging conference, SPIE Photonics West, März 2021), virtuell.

B. Kemper, H. Eilers, T. Klein, K. Brinker, S. Ketelhut, "Quantitative phase imaging-based machine learning approaches for the analysis of adherent and suspended cells", Quantitative phase imaging conference, SPIE Photonics West, März 2021, virtuell.

K. Lamann, D. Breitenstein, C. Brungs, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Analysis of Tattooed Human Skin Samples by ToF-SIMS and Orbitrap-SIMS. SIMS, Oktober 2019, Kyoto, Japan.

K. Lamann, D. Breitenstein, C. Brungs, E. Tallarek, A. Pirkl, E. Niehuis, U. Karst, B. Hagenhoff: Analysis of Tattooed Human Skin Samples by ToF-SIMS and Orbitrap-SIMS. 1 st Winter School on Advanced Materials, Metrology and Standardization, Februar 2020, Claviere, Italien.

A. Ntovas, Á. Barroso, K. Eder, S. Ketelhut, A. Vennemann, M. Wiemann, J. Schnekenburger, B. Kemper, "Utilizing quantitative phase imaging with digital holographic microscopy for characterization of nanomaterial-induced tissue alterations", Quantitative phase imaging conference, SPIE Photonics West On Demand, Februar 2022, virtuell.

A. Ntovas, Á. Barroso, K. Eder, A. Vennemann, M. Wiemann, J. Schnekenburger, B. Kemper, "Quantification of Nanoparticle-mediated Alterations in Histological Tissue Sections Utilizing Quantitative Digital Holographic Phase Microscopy", OSA Digital Holography and Three-Dimensional Imaging, June 2021, virtuell.

I. Nordhorn, C. Verlemann, A. Vennemann, M. Sperling, M. Wiemann, U. Karst: LA-ICP-MS based methods for the analysis of gold nanoparticles in rat spleen and kidney tissues. CANAS, September 2019, Freiberg, Deutschland.

J. Schnekenburger: Label free optical cell and tissue characterization, 17th European Congress of Toxicologic Pathology, September, 2019, Köln, Deutschland.

J. Schnekenburger: Quantitative Phase Imaging for Cellular Process Characterization and Cell Quality Control, 46th International Conference of the European Society of Artificial Organs, September 2019, Hannover Germany.

S. Seiffert, I. Nordhorn, L. Ma-Hock, S. Gröters, S. Kröger, O. Hachmöller, M. Wiemann, U. Karst: Laser ablation-ICP-MS as an alternative method to determine spatially resolved and quantitatively the organ burden directly in histological sections, 7th German Pharm-Tox Summit, 7-10 Mar 2022 (online conference).

S. Seiffert, A. Vennemann, E. Niehaves, S. Kröger, M. Wiemann, U. Karst: A calibration approach to size and localize CeO2 nanoparticles in remote organs using microsecond LA-spICP-MS, SciX 2021, 26 Sep - 1 Oct 2021 (online participation).

S. Seiffert, A. Vennemann, E. Niehaves, S. Kröger, M. Wiemann, U. Karst: Localization and sizing of CeO2 nanoparticles in remote organs using LA-spICP-MS DGMS Young Scientist 1st Digital Meeting, 17 - 18 Jun 2021.

S. Seiffert, A. Vennemann, S. Kröger, M. Wiemann, U. Karst: Focal accumulation in remote organs: a quantitative LA-ICP-MS study, NanoTox 2021, 20 - 22 Apr 2021 (online conference).

S. B. Seiffert, A. Vennemann, E. Niehaves, S. Kröger, N. Honarvar, M. Wiemann, U. Karst: A calibration approach for the sized-resolved analysis of CeO2 in tissue sections and cells, 6. Doktorandenseminar des DAAS, 20 - 21 Sep 2021 (online conference).

M. Wiemann: Quantifizierung von Nanomaterialien im Gewebe für die regulatorische Analytik und Entwicklung von in vitro-Methoden. NanoCare Clustermeeting, Frankfurt, 26.9.2019.